SeedGenes Profile: PDE 326

Gene Information Gene Symbol PDE 326 Chromosome Locus At3g03710   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol RIF 10 (M. Rodgriguez-C) Sequence Identity Gene Class Seed Pigment Predicted Function Plastid Polynucleotide Phosphorylase Function Details Proposed role in 23S rRNA 3' end processing Gene Evidence Border Recovery Meinke / Salk (2 alleles) Border Recovery Rodriguez-Concepcion / Weigel (1 allele) Border Recovery Rodriguez-Concepcion / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 5797 nucleotides mRNA: 3103 nucleotides Protein:   922 amino acids cDNA Status FLcDNA Available: AJ252123 Top BLAST Hits Arabidopsis: AT5G14580.1 (42%) Plant ESTs: Rice (Oryza sativa) (73%) Model Genomes:   Escherichia coli (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 259344_at

Mutant Information Allele Symbol pde 326-1 pde 326-2 rif 10-1 rif 10-3 Mutant Class Seed Pigment Seed Pigment Seed Pigment Seed Pigment Source of Mutant Meinke / Salk Meinke / Salk Rodriguez-Concepcion / Weigel Rodriguez-Concepcion / Salk Mutant Line Number SALK_070705 SALK_037353 N/A SALK_013306 Ecotype Columbia Columbia Columbia Columbia Genetic Background         ABRC Stock         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence         Location of Mutation Exon 12 Intron 5 Intron 12 Intron 3 Terminal Phenotype Pigment Defective  Details Pigment Defective  Details Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Sequence Identity Database Release December, 2010 December, 2010 December, 2010 December, 2010 Seed Color Pale Green Pale Green     Embryo Color Pale Green Pale Green     Percent Mutant Seeds 27.1 % 27.9 %     Chi - Square 0.6 1.0     Seeds Counted 251 222     Percent Top Half 48.5 % 59.7 %     Chi - Square 0.1 2.3     Average Seed Length 550 ± 0 µm 550 ± 0 µm     Average Embryo Length 400 ± 0 µm 400 ± 0 µm