SeedGenes Profile: HISN 2

Gene Information Gene Symbol HISN 2 Chromosome Locus At1g31860   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the second step in histidine biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (3 alleles) Database Release October, 2007 Predicted Sequences Gene: 2500 nucleotides mRNA: 1065 nucleotides Protein:   281 amino acids cDNA Status FLcDNA Available: AY086514 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (86%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 246265_at

Mutant Information Allele Symbol hisn 2-1 hisn 2-2 hisn 2-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Meinke / Salk Mutant Line Number SALK_047603 SALK_143868 SALK_055503 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock       Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence       Location of Mutation Exon 5 Exon 5 5' UTR Terminal Phenotype Preglobular  Details Preglobular  Details Cotyledon  Details Nomarski Phenotype Preglobular  Images Preglobular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 October, 2007 Seed Color White White White to Pale Yellow Embryo Color White White White to Pale Yellow Percent Mutant Seeds 16.0 % 16.0 % 23.9 % Chi - Square 38.5 72.7 1.3 Seeds Counted 898 1672 2117 Percent Top Half 71.5 % 69.3 % 55.9 % Chi - Square 26.7 39.7 7.1 Average Seed Length 320 ± 80 µm 220 ± 80 µm 340 ± 100 µm Average Embryo Length 20 ± 20 µm 15 ± 0 µm 110 ± 140 µm