SeedGenes Profile: RML 1

Gene Information Gene Symbol RML 1 Chromosome Locus At4g23100   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol CAD 2 (C. Cobbett) Sequence Identity Gene Class Embryo Defective Predicted Function Gamma-Glutamylcysteine Synthetase Function Details First enzyme in glutathione biosynthesis; required for initiation and maintenance of cell division Gene Evidence Sequenced Mutant Allele R. Sung (1 allele) Molecular Complementation R. Sung (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4139 nucleotides mRNA: 2055 nucleotides Protein:   522 amino acids cDNA Status FLcDNA Available: AY056372 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 254270_at

Mutant Information Allele Symbol rml 1-1S Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 23012 Ecotype Columbia Genetic Background   ABRC Stock CS16066 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 7 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Pale Yellow-Green Embryo Color Pale Yellow-Green Percent Mutant Seeds 26.2 % Chi - Square 0.9 Seeds Counted 1108 Percent Top Half 49.1 % Chi - Square 0.1 Average Seed Length 500 ± 40 µm Average Embryo Length 390 ± 70 µm