SeedGenes Profile: EMB 2411

Gene Information Gene Symbol EMB 2411 Chromosome Locus At1g08840   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Helicase/Nuclease (Dna2) Function Details DNA replication; Okazaki fragment metabolism Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 8791 nucleotides mRNA: 3891 nucleotides Protein:   1296 amino acids cDNA Status Unknown Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (54%) Model Genomes:   Neurospora crassa (34%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264801_at

Mutant Information Allele Symbol emb 2411-2 Mutant Class Embryo Defective Source of Mutant Meinke / Genoplante Mutant Line Number DYE165 Ecotype Wassilewskija Genetic Background   ABRC Stock CS24093 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 20 Terminal Phenotype Preglobular  Details Nomarski Phenotype Globular  Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 28.3 % Chi - Square 5.1 Seeds Counted 876 Percent Top Half 58.5 % Chi - Square 7.1 Average Seed Length 490 ± 70 µm Average Embryo Length 20 ± 0 µm